Cite this article as:
Kulapina E. G., Kulapina O. I., Anikina V. D. The Research of the Behavior of Cefixime in Aquatic and Biological Media by Spectrophotometric Method. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2020, vol. 20, iss. 1, pp. 10-15. DOI: https://doi.org/10.18500/1816-9775-2020-20-1-10-15
The Research of the Behavior of Cefixime in Aquatic and Biological Media by Spectrophotometric Method
The research of behavior of cefixime in biological media was conducted by spectrophotometric method. It was shown that the maximum of light absorption of cefixime against the background of oral fluid is observed at λ = 292 nm and for blood serum at λ = 289 nm. The interval of linear dependence optical density – concentration of cefixime is 3–50 mkg/ml. The optimal conditions of precipitation of proteins in the studied biological media were revealed. It was shown that the correlation between the maximum wavelength (λmax, nanometer) and the optical density (Amax) for absorption spectra of cefixime is observed in blood serum (with additional precipitation of proteins) and oral liquid (with precipitation of proteins), i.e. behavior of an antibiotic in the studied biological media is similar. This fact indicates the possibility of identification of cefixime in oral fluid of patients. The behavior of cefixime in aquatic media is investigated to assess the possibility of using spectrophotometric identification of cefixime in medicines. Ranges of linearity and the limit of detection of an antibiotic are determined. Influence of acidity of media on spectroscopic characteristics of cefixime was revealed. It was found that aqueous solutions of an antibiotic have various acidity рН 2.90–5.04 depending on concentration 10–50 mkg/ml. The possibility of spectrophotometric identification of cefixime in aquatic and biological media was shown.
1. Yakovlev V. P. Antibacterial Therapy. Antibiotics and Chemother., 2003, vol. 48, no. 17, pp. 3‒4.
2. Egorov N. S. Osnovy ucheniya ob antibiotikakh [The Bases of Doctrine about Antibiotics]. Moscow, Nauka Publ., 2004. 528 p. (in Russian).
3. Ahmed S. M. A., Elbashir A. A., Aboul-Enein H. Y. New Spectrophotometric Method for Determination of Cephalosporins in Pharmaceutical Formulations. Arab. J. Chem., 2011, vol. 76, pp. 332‒346.
4. El-Shaboury S. R., Mohamed F. A., Saleh G. A., Rageh A. H. Analysis of Cephalosporin Antibiotics. J. Pharm. Biomed. Anal., 2007, vol. 45, pp. 1–19.
5. Shah J., Rasul M. J., Shah S. Spectrofl uorimetric Method for Determination and Validation of Cefi xime in Pharmaceutical Preparations Through Derivatization with 2-cyanoacetamide. J. Fluores, 2011, vol. 21, no. 2, pp. 579‒585. 6. Jain R., Gupta V. K., Jadon N., Radhapyari K. Voltammetric Determination of Cefi xime in Pharmaceuticals and Biological Fluids. Anal. Biochem., 2010, vol. 407, no. 1, pp. 79‒88.
7. Ojani R., Raoof J.-B., Zamani S. A novel Sensor for Cephalosporins Based on Electrocatalytic Oxidation by Poly(o-anisidine)/SDS/Ni Modifi ed Carbon Paste Electrode. Talanta, 2010, vol. 81, no. 4‒5, pp. 1522‒1528.
8. Kondratenko S. N., Starodubtsev A. K., Zolkina I. V., Kovacevic I. V., Kondratenko N. A., Senik B. A. Techniques of Modeling of Pharmacokinetics of Some Medicines on Dynamics of Their Distribution in Saliva. Biomedical Chemistry, 2014, vol. 60, no. 2, pp. 221‒222 (in Russian).
9. Rebrova O. Y. Statisticheskiy analiz meditsinskikh dannykh [Statistical Analysis of Medical Data]. Moscow, MediaSphera Publ., 2002. 312 p. (in Russian).
10. Alekseev V. G. Bioneorganicheskaya khimiya penitsillinov i tsefalosporinov [Bioinorganic Chemistry of Penicillin and Сefalosporin]. Tver, Tver. gos. un-t, 2009. 104 p. (in Russian).
