Cite this article as:

Tumskaia A. V., Kosyreva I. V. Express determination of cephalexin. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2021, vol. 21, iss. 3, pp. 260-266. DOI: https://doi.org/10.18500/1816-9775-2021-21-3-260-266

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Heading: 
Chemistry
UDC: 
543.068.8
Language: 
Russian

Express determination of cephalexin

Authors: 
Tumskaia Anastasiia Vyacheslavovna, Saratov State University , 83, Astrakhanskaya str., Saratov, 410012, Russia , marakaeva_anastasiya@mail.ru
Kosyreva Irina Vladimirovna, Saratov State University , 83, Astrakhanskaya str., Saratov, 410012, Russia , i_kosyreva@mail.ru
Тип статьи для РИНЦ: 
RAR научная статья
Abstract

Test means based on immobilized Fehling’s reagent for the determination of cephalexin have been obtained. Methods for visual and colorimetric assessment of antibiotic concentration using a smartphone camera have been developed. The optimal conditions for the indicator reaction have been selected with varying heating time and temperature. For a visual semi-quantitative assessment of the content of cephalexin, a color scale has been obtained. The metrological characteristics of the test method have been determined: the range of the determined contents is 0.5–16 mg/ml, the unreliability interval is 0.1–0.4 mg/ml, and the detection limit is 0.4 mg / ml. For the colorimetric assessment of the concentration of cephalexin, a linear dependence of the intensity of the Blue channel on the logarithm of the concentration of cephalexin (у = –96x + 144, r 2 = 0.99) has been constructed, and linear dependences of the area (у = –36986x + 62458, r 2 = 0.96) and perimeter (у = –270x + 786, r 2 = 0.93) from the logarithm of the concentration of cephalexin have been obtained. The range of the determined contents was 0.1–16 mg/ml, the lower limit was 0.1 mg / ml. The verification of the correctness of the developed test methods was carried out by the «introduced-found» method (Sr  0.13).

Key words: 
express determination, test tools, indicator papers, cephalexin, colorimetry
DOI: 
https://doi.org/10.18500/1816-9775-2021-21-3-260-266
References
  1. Sahm D. F., Thornsberry C., Jones R. N. β-Lactam antibiotics: The fi rst- and second-generation cephalosporins. Antimicrob. Newsl., 1985, vol. 2, no. 4, pp. 25–28.
  2. Ob utverzhdenii perechnya zhiznenno neobhodimyh i vazhnejshih lekarstvennyh preparatov na 2018 g.: Rasporyazhenie Pravitel’stva RF ot 23 oktyabrya 2017 g. № 2323-r. [Order of the Government of the Russian Federation October 23, 2017 No. 2323-p “On approval of the list of vital and essential medicines for 2018”]. Available at: government.ru>docs>29758
  3. Wick W. E. Cephalexin, a new orally absorbed cephalosporin antibiotic. Applied Microbiology, 1967, vol. 15, no. 4, pp. 765–769.
  4. Solberg C. O., Schreiner A., Digranes A. Cephalexin therapy of lower respiratory tract, soft tissue and bone infections. Scand. J. Infect. Dis., 1972, vol. 4, no. 3, pp. 241–243.
  5. Oliveira R. V., De Pietro A. C., Cass Q. B. Quantifi cation of cephalexin as residue levels in bovine milk by highperformance liquid chromatography with on-line sample cleanup. Talanta, 2007, vol. 71, no. 3, pp. 1233–1238.
  6. Coran S. A., Bambagiotti-Alberti M., Giannellini V., Baldi A., Picchioni G., Paoli F. Development of a densitometric method for the determination of cephalexin as an alternative to the standard HPLC procedure. Journal of Pharmaceutical and Biomedical Analysis, 1998, vol. 18, no. 1-2, pp. 271–274.
  7. Wu S. G., Lai E. P. C., Mayer P. M. Molecularly imprinted solid phase extraction–pulsed elution–mass spectrometry for determination of cephalexin and α-aminocephalosporin antibiotics in human serum. Journal of Pharmaceutical and Biomedical Analysis, 2004, vol. 36, no. 3, pp. 483–490.
  8. Chen S., Li Y., Wu S., Jiang X., Yang H., Su X., Yang Y. A phosphorescent probe for cephalexin consisting of mesoporous thioglycolic acid-modifi ed Mn:ZnS quantum dots coated with a molecularly imprinted polymer. Microchim. Acta, 2020, vol. 187, no. 1, pp. 40.
  9. Chen L., Wang Z., Ferreri M., Su J., Han B. Cephalexin residue detection in milk and beef by ELISA and colloidal gold based one-step strip assay. J. Agric. Food. Chem., 2009, vol. 57, no. 11, pp. 4674–4679.
  10. Patel S. A., Patel N. M., Patel M. M. Spectrophotometric methods for the estimation of Cephalexin in tablet dosage forms. Indian Journal of Pharmaceutical Sciences, 2006, vol. 68, pp. 278–280.
  11. Elbashir A. A., Ahmed S. M. A., Suliman F. E. O. A novel spectrophotometric for the determination of cephalosporins using 8-hydroxy-1, 3, 6-pyrenetrisulfonic acid trisodium salt (HPTS) as a chromogenic reagent. American Academic & Scholarly Research Journal, 2012, vol. 4, no. 4, pp. 1–13.
  12. Amin A., Shama S. Vanadophosphoric Acid as a Modifi ed Reagent for the Spectrophotometric Determination of Certain Cephalosporins and their Dosage Forms. Monatshefte für Chemie, 2000, Bd. 131, S. 313–319.
  13. Arzamastsev A. P. Farmatsevticheskaya khimiya [Pharmaceutical Chemistry]. Moscow, GEOTAR-MED Publ., 2004. 640 p. (in Russian).
  14. Moratal J. M., Borrás J., Donaire A., Martínez M. J. Coordinating properties of the cephalexine antibiotic. A potentiometric study of the complexes formation between cephalexine and Co (II), Ni (II) and Cu (II) metal ions. Inorg. Chim. Acta, 1989, vol. 162, no. 1, pp. 113–119.
  15.  Anacona J. R., Rodriguez I. Synthesis and antibacterial activity of cephalexin metal complexes. J. Coord. Chem., 2004, vol. 57, no. 15, pp. 1263–1269.
  16. El-Maali N. A. Voltammetric analysis of Cu (II), Cd (II) and Zn (II) complexes and their cyclic voltammetry with several cephalosporin antibiotics. Bioelectrochemistry, 2005, vol. 65, no. 2, pp. 95–104.
  17. Feigl F. Spot Tests in Organic Analysis. New York, Elsevier Publishing Company, 1960. 675 р.
  18. Kholin Yu. V., Nikitina N. A., Panteleimonov A. V., Reshetnyak E. A., Bugaevsky A. A., Loginova L. P. Metrologicheskiye kharakteristiki metodik obnaruzheniya s binarnym otklikom [Metrological Characteristics of Detection Methods with a Binary Response]. Kharkov, Timchenko Publ., 2008. 128 p. (in Russian).
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