Cite this article as:
Zudina I. V., Vedyaeva A. P., Bulkina N. V., Ivanov P. V., Alzubaidi A. F. In Vivo and in Vitro Studies of the Effect of Chitosan on the Bone Defect Repair Process . Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2016, vol. 16, iss. 2, pp. 171-179. DOI: https://doi.org/10.18500/1816-9775-2016-16-2-171-179
In Vivo and in Vitro Studies of the Effect of Chitosan on the Bone Defect Repair Process
The objective of our in vivo and in vitro studies was to examine the molecular and cellular mechanisms of the anti-inflammatory and wound-healing effects of chitosan barriers when used for guided tissue regeneration (GTR) in a rabbit model. An ELISA test of the rabbit blood serum conducted during the treatment of alveolar bone defects has confirmed the ability of chitosan to reduce inflammation fast by suppressing the TNF- cytokine production. Our in vitro data show that chitosan stimulates fibroblasts and epithelial cells to synthesize the vascular endothelial growth factor (VEGF) and the granulocyte-macrophage colony-stimulating factor (GM-CSF) in the early post-surgery period. This could have important clinical implications because the success of flap surgery largely depends on the time of initiation of angiogenesis and neovascularization.
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