Cite this article as:

Kovrizhnikov A. V., Pylaev T. E., Zakharevich A. M., Konnova S. A., Kupryashina M. A. Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2021, vol. 21, iss. 3, pp. 298-303. DOI: https://doi.org/10.18500/1816-9775-2021-21-3-298-303

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Heading: 
Biology
UDC: 
577.151
Language: 
Russian

Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel

Authors: 
Kovrizhnikov Aleksandr Viktorovich, Saratov State University , 83, Astrakhanskaya str., Saratov, 410012, Russia , alexander.kovrizhnikov@gmail.com
Pylaev Timofey Evgenievich, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences, 410049, Russia, Saratov, Entuziastov Avenue, 13, pylaev_t@ibppm.ru
Zakharevich Andrey Machailovich, Saratov State University , 83, Astrakhanskaya str., Saratov, 410012, Russia , zaharevicham@yandex.ru
Konnova Svetlana Anatolyevna, Saratov State University , 83, Astrakhanskaya str., Saratov, 410012, Russia , konnovasa@yandex.ru
Kupryashina Maria Alexandrovna, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences, 410049, Russia, Saratov, Entuziastov Avenue, 13, kupryashina_m@mail.ru
Тип статьи для РИНЦ: 
RAR научная статья
Abstract

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding. We demonstrated the retained respiratory activity and growth ability of the bacteria during immobilization, thus confirming the advantageous prospects of alginate templates for azospirilla encapsulation

Key words: 
Azospirillum, alginate, immobilization, encapsulation, viability
DOI: 
https://doi.org/10.18500/1816-9775-2021-21-3-298-303
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himiya_2021_3_298-303.pdf