This study was done to evaluate the bacterial profile and antibiotic susceptibility pattern of urinary tract infections (UTIs) microbial causative agents. For proper identification of causative microbial agents, mid– stream urine (MSU) samples were taken from 325 patients suspected to have UTI. These specimens were cultured and subjected to appropriate biochemical tests. Our results revealed that 200 urine samples showed positive cultures. The most prevalence isolates were Escherichia coli with frequency rate of 55.5%, followed by Klebsiella spp. (14%), Enterobacter spp.