The specifity of the formation of arachinic acid monolayers (used as models of cell membranes) by the Langmuir method on a subphase containing L-ascorbic and D-isoascorbic acids with a concentration of 10-2, 10-3 M at a temperature of 25, 37 and 41°C was studied. The influence of the isoforms of ascorbic acid, the composition and temperature of the subphase on the mechanical properties of monolayers (specific area, compressibility, collapse pressure) was experimentally found.