The ability of using cellulose diacetate (CDA) as matrix for luminescent determination of polycyclic aromatic hydrocarbons (PAH) in aqueous media was studied. PAH sorption was carried out on the example of pyrene from water solutions. Cellulose diacetate matrix was modified by anionic and cationic surfactants - sodium dodecylsulphate (SDS) and cetyltrimethylammonium bromide (CTAB). The comparative analysis of dependence of fluorescence intensity and polarity index of pyrene was carried out.