альгинат

Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel

Authors: Kovrizhnikov A. V., Pylaev T. E., Zakharevich A. M., Konnova S. A., Kupryashina M. A.

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding.

Preparation and Identification of the Molecular Properties of Alginate Synthesized by the Cultivation of Azotobacter vinelandii D-05

Authors: Shutova V. V., Rusyaeva A. B.

It is known that bacterial alginates are widely used in biomedicine as carriers in the immobilization of cells, enzymes and drugs. Their functional properties depend on their monomeric composition and molecular weight and vary depending on the source and the cultivation conditions. It was established that molasses (waste of sugar beet production) can be used as an effective and cheap source of nutrition for the growth of the bacterium Azotobacter vinelandii strain D-05 and the production of alginate. Submerged periodic cultivation of the producer on media of different composition was used.