It is shown that in the analysis of medicines fluoroquinolone by reversed-phase HPLC with fluorimetric detection modification mobile phase γ-cyclodextrin leads to a decrease in the detection limit of antibiotics to 8 times. The possibility of determination of cipro- floxacin in the presence of tetracycline and quinolone derivative series. The proposed method is tested in the analysis of pharmaceutical preparations containing ciprofloxacin.