Biology

Developing the Algorithm of Increasing the Destructive Potential of Pseudomonas putida SU12 Native Strain Extracted From Phenol-Contaminated Soils

Authors: Ilyina N. A., Ksenofontova O. Y., Tretyakova S. E., Tikhomirova E. I.

The article presents the development of methodological approaches to increasing destructive potential of Pseudomonas putida SU12 native strain extracted from phenol-contaminated soils. The cyclic selective method on gradual increase of phenol concentration in optoelectronics environment in combination with intermediate stage of accumulative culture is described. The authors proposed a discrete, stepwise increase in the concentration of phenol in elective media depending on degree of its destruction.

Biometric Characteristics and Aerodynamic Properties of Pollen Grains of North American Pines under Conditions of the Southern Coast of the Crimea

Authors: Plugatar Y. V., Sakhno T. M.

The results of the study of biometric characteristics and aerodynamic properties of pollen of North American species: Pinus radiata D. Don, Pinus sabiniana Douglas, Pinus coulteri D. Don and autochthonous Pinus nigra subsp. pallasiana (Lamb.) Holmboe, grown on the Southern Coast of the Crimea (SCC) are presented. The differences in the size of the pollen grains and their individual parameters are revealed. The pollen of introduced species is characterized by large sizes in comparison with native species.

Preparation and Identification of the Molecular Properties of Alginate Synthesized by the Cultivation of Azotobacter vinelandii D-05

Authors: Shutova V. V., Rusyaeva A. B.

It is known that bacterial alginates are widely used in biomedicine as carriers in the immobilization of cells, enzymes and drugs. Their functional properties depend on their monomeric composition and molecular weight and vary depending on the source and the cultivation conditions. It was established that molasses (waste of sugar beet production) can be used as an effective and cheap source of nutrition for the growth of the bacterium Azotobacter vinelandii strain D-05 and the production of alginate. Submerged periodic cultivation of the producer on media of different composition was used.

Bacteria Fouling in the Systems of Technical and Circulating Water Supply of Balakovo Nuclear Power Station

Authors: Kirilova A. V., Glinskaya E. V., Voronin M. Y., Saveleva A. S.

The article is devoted to the study of bacteria in the system of technical water supply of Balakovo nuclear power plant (BNPP). The work was carried out in 2017. The samples were taken at the following sites: backup diesel generator of the 3rd unit (røde’s-3), pumping station of feeding pond-cooler (PSFPC), coastal pump station of unit 3 (CPS-U3), coastal pumping station of power unit № 4 (CPSP-U4), turbine hall of power unit № 3 (TH-PU3), turbine hall of power unit № 4 (TH-PU4).

Development of Fluorescent Glioma in Rats under Pharmacological Modulation of Beta2-Adrenergic Receptor

Authors: Khorovodov A. P., Shirokov A. A., Novolokin N. A., Terskov A. V., Lauberts E. A., Mamedova A. T., Shushunova N. A., Agranovich I. M., Ulanova M. V., Bodrova A. A., Semyachkina-Glushkovskaya O. V.

This study shows the role of adrenergic mechanisms in the development of fluorescent glioma in rats and the impairment of the blood-brain barrier (BBB) permeability. The results demonstrate that the progression of glioma was accompanied by a gradual increase in the BBB permeability and an increased expression of vascular beta2-adrenoreceptors (B2-AR). The pharmacological blockade of B2-AR reduced the degree of BBB disruption, the migration of cancer cells and increased the survival of animals.

The Effect of Pollutants on the Emergence of Rifampicin Resistance in Bacteria of the Pseudomonas Genus

Authors: Seliverstova E. Y., Vyrostkov V. A., Yarovaya E. V., Kulikov M. P., Drobot V. Y., Sazykin I. S., Sazykina M. A.

The anthropogenic pressure on ecosystems may contribute to the spread of antibiotic resistance among bacteria in natural conditions. Due to this, the aim of this work was to study the impact of specific biocides, antibiotics, pesticides and heavy metals on the emergence of rifampicin resistant mutants of Pseudomonas putida. To assess the impact of toxic substances on the emergence of rifampicin-resistant mutants the studied substances and rifampicin in the final concentration of 100 μg/ml were added into nutrient medium before plating.

Lymphatic Meningeal Role in Processes of Brain Clearing: in vivo Visualization

Authors: Duarte Torres E. N., Abdurashitov A. S., Namykin A. A., Shirokov A. A., Shushunova N. A., Sarantseva E. I., Semyachkina-Glushkovskaya O. V.

In the experiments on rats, Evans Blue dye and gold nanorods were injected into the brain parenchyma, followed by visualization with optical coherent tomography and a fluorescent microscopy. The results showed that meningeal lymphatics play an important role in the brain clearing, using markers introduced into the tissues. It has been shown that the deep cervical lymph node is the first anatomical “station” for the outflow of fluid from the brain. The data obtained shed light on the lymphatic mechanisms underlying the drainage and clearing of brain functions.

Phenological Characteristics of Orthopterous Insects (Orthoptera) in the Volga-Kama State Nature Biosphere Reserve

Authors: Karmzina I. O., Shulaev N. V.

In this brief communication, the results of the research on the seasonal dynamics of orthopterous insects (Orthoptera) are discussed. The research was performed in the Volga-Kama State Nature Biosphere Reserve. Field sampling and observations were carried out for different biotopes of the nature reserve. The phenological characteristics of 26 background species of the families Tettigonioidea, Tetrigidae, and Acridoidea were revealed. The species under study were divided into five phenological groups: spring-summer, early summer, summer summer-late summer, and late summer.

Optimization of Photodynamic Effects on Permeability of Cerebral Vessels

Authors: Semyachkina-Glushkovskaya O. V., Shirokov A. A., Novolokin N. A., Terskov A. V., Bodrova A. A., Shushunova N. A., Khorovodov A. P., Ulanova M. V., Agranovich I. M., Iskra T. D., Mamedova A. T.

Photodynamic treatment (PDT) causes a significant increase in the permeability of the blood-brain barrier (BBB) in healthy mice. Using different doses of laser radiation (635 nm, 10–40 J/cm2 ) and photosensitizer (5-aminolevulinic acid – 5-ALA, i.v.), we found the optimal PDT for the reversible opening of the BBB, exhibiting brain tissue recovery 3 days after PDT. Further increases in the laser radiation or 5-ALA doses have no amplifying effect on the BBB permeability, but are associated with severe damage of brain tissues.

Epiphytic Lichens of Natural Park “Kumysnaya Polyana”

Authors: Kozyreva E. A., Boldyrev V. A.

The article provides information about the epiphytic lichen flora of the Natural Park “Kumysnaya Polyana” (NP), which is located in Saratov. It is a regional SPNA (Special Protected Natural Area). The researches were conducted in forest communities of NP in 2011–2016. Standard techniques were used in the collection and identification of lichens. An index of species specificity of lichens for forophytes was calculated. An annotated list consists of 37 species and includes the information about their preferential forophytes.

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