Biology

Peculiarities of Modulation by Arginine-vasopressin the Regular and Non-Regular Spike Activity of the Suprahiasmtic Nucleus Neurons

Authors: Tkacheva М. А., Karyan S. D., Inyushkina Е. М., Inyushkin А. N.

The suprachiasmatic nucleus generating biological circadian rhythms in mammals and humans, contains a high proportion of neurons, producing arginine-vasopressin. In in vitro experiments on hypothalamic slices of rats, the comparative analysis of the effect of 20 nM argininevasopressin on the spike activity parameters of neurons with regular (n =23) and irregular (n=28) activity in the suprachiasmatic nucleus was performed.

The Use of Malaise Trap for the Study of the Beetles Fauna (Insecta: Coleoptera) in the Territory of National Park «Khvalynsky» Saratov Province

Authors: Sazhnev A. S., Anikin V. V.

In the article results of use of Malaise trap for the study of the beetles local fauna (Insecta: Coleoptera) in the territory of National Park «Khvalynsky» is given. Compiled a list of 95 species from 32 families Coleoptera. Antherophagus pallens (Linnaeus, 1758) and Saperda octopunctata (Scopoli, 1772) are recorded for Saratov region in first time.

Laser-induced Vasomotor Responses on Chorioallantoic Membrane

Authors: Postnov D. E.

The strength and the specific featu res of the vasomotor responses provide important information about the healthy state of the blood vessel, since it s contraction or dilation is caused by the activation or relaxation of vascular smooth muscle cells of the vascular wall and appears to be a universal response to the a number of physiological regulatory pathways, including humoral and neurogenic ones.

Translocation of Copper, Zinc, Iron and Manganese in the Cultivated Oat (Avena sativa L., 1753)

Authors: Petukhov А. S., Khritokhin N. А., Petukhova G. А., Kudryavtsev А. А.

Due to rising anthropogenic pressure, heavy metal pollution of the environment becomes the significant danger. This study is devoted to the investigation of Cu, Zn, Fe and Mn concentration in the oat and two types of soil, model polluted by metal sulfates. The method of atomic absorption spectroscopy was used. Cu concentration in mineral soil was much higher than Zn. When Cu and Zn were combined, also Fe and Mn, pairwise antagonism in mineral soil and in the oat was discovered. The oat accumulates Cu and Mn more actively than Zn and Fe.

Special Features of Flowering in Some Clematis L. Species and Forms from Nikita Botanical Gardens Collection

Authors: Zubkova N. V.

In the studies of ornamental plants seasonal development, flowering rhythms are exceptionally important, since the main criteria for the ornamental value of plants used for landscaping, are the time and duration of their flowering. The aim of the present work was to identify special features of flowering in some Clematis L. species and forms from Nikita Botanical Gardens collection. The objects of this study were 16 taxa. The methodology was based on the principles of introductory studies. Phenological and mathematical methods were applied.

Rhizosphere Microorganisms’ Collection of IBPPM RAS: Revision of Azospirillum Strains Based on 16S rRNA Gene Sequence Analysis

Authors: Turkovskaya O. V., Golubev S. N., Dubrovskaya, E. V.

The IBPPM Collection maintains unique pool of bacterial strains related to Azospirillum genus, notable for its biotechnological potential. Progressively developing systematics of this genus requires checking and clarifying the taxonomic position for long-term stored isolates in the collection. Consequently, the species membership was confirmed or defined in the tested Azospirillum strains using a comparative analysis of their full-length 16S rRNA genes combined with morphological, cultural, physiological and biochemical properties, as well as DNA-DNA hybridization data.

Effect of N,O,S-contaning Heterocyclic Compounds on Growth of Root System of Triticum aestivum L.

Authors: Korobko V. V., Samsonova Е. А., Batalin S. D., Pchelintseva N. V., Luneva M. А.

Conducted biological testing of synthetic heterocyclic compounds: 2-benzoylmethyl-3-benzoyl-2-phenylaziridine, 2-benzoyl-3, 5-diphenyl-4-chlorofuran and 2-amino-4(1,3-diphenyl-2-chloropropene-1-one-3-yl)-5-phenyl-1,3-thiazole. The investigated compounds are members of three series of heterocyclic compounds and obtained at the Department of Organic and Bioorganic Chemistry National Research Saratov State University. The concentration of substances established by the molecular weight, in the three specific to physiologically active substances acting doses: 10-6 М, 10-9 М, 10-12 М.

Antimycotic Activity of Silver Nanoparticles Depending on the Stabilizer Used

Authors: Shulgina T. A., Nechayeva O. V., Glinskaya E. V., Torgashova A. S., Teslyuk D. A., Babailova A. V., Panfilova E. A.

In the last decade, a large number of factual data about the steadily increasing complications caused by gram-positive and gram-negative microorganisms, as well as caused by fungi of the genus Candida, have been obtained. The developed situation was a powerful stimulus for the search and creation of new products containing substances with pronounced chemical-physical properties and high biological activity. The modern and promising direction was the using of nano- technology, in particular, metallic nanoparticles.

Mosquitos (Diptera; Culicidae) of the Saratov Region

Authors: Knyazeva Т. V., Porshakov A. M., Zakharov К. S., Matrosov А. N., Tolokonnikova S. I., Chekashov V. N., Shilov М. М., Yakovlev S. A.

Described is the fauna of bloodsucking mosquitoes found in the Saratov Region. The article is based on the data collected dur- ing the field surveys in the flooded biotopes along the Volga and Don river basins and Kamysh-Samara enclosed lake basin, as well as on the literature data available. By 1970s, 31 species of mosquitoes have been spotted in the Region. The paper presents qualitative and quantitative changes over the 45-year period in the populations of bloodsucking mosquito, habitant in the flooded areas of different rivers.

Conserved and Variable Regions of the Methanol Dehydrogenase Gene Among Halotolerant Representatives of Methylophaga Genus

Authors: Sharavin D. Y., Solomennyi А. P.

We investigated conserved and variable regions of the mxaF gene and amino acid sequence in methylotrophic strain M1K isolated from saline soil rhizosphere and belonging to Methylophaga genus. The significant heterogeneity was revealed in methano- and methylotrophic bacteria by method of Minimum Evolution. The ability of strain to influence indole-3-acetic acid synthesis from L-tryptophan and halotolerance are the arguments in favor of application in new agrotechnology.

Pages