Biology

Peculiarities of the Structure of Azospirillum zeae and Azospirillum formosense Lipopolysaccharides

Authors: Potapova О. А., Sigida E. N., Konnova S. A.

Lipopolysaccharides (LPS) of plant-growth-promoting bacteria of the genus Azospirillum are involved in the initial steps of formation of the association with host plant. This work aimed compositional analysis of the LPS from Azospirillum zeae and Azospirillum formosense. The LPS were extracted from the cells by hot phenolwater solution. Biopolymer composition of the LPS was studied by colorimetric methods. Determination of the fatty acid composition of the lipid A was performed by GLC. O-deacylation of the LPS A.

Study of Phylogenetic Connections of Representatives of the Genus Mesobuthus (Arachnida: Scorpiones) on the Basis of Molecular DNA Analysis

Authors: Poverenniy N. N., Anikin V. V.

The aim of the research is to determine the phylogenetic connections of scorpion representatives of the genus Mesobuthus living in the Lower Volga region and their comparison with other taxa (subspecies and species of this genus). For this, scorpions were collected on the territory of the Lower Volga region. The nucleotide sequences of the collected specimens were deciphered and compared with the sequences of species of the genus Mesobuthus from other regions of the Palearctic. The phylogenetic connections of the investigated objects are determined.

The Involvement of Laccase of the Fungus Stropharia Rugosoannulata DSM 11372 in Pah Degradation

Authors: Balandina S. А., Pozdnyakova N. N., Turkovskaya O. V.

Soil-inhabiting ligninolytic basidiomycetes, such as Stropharia rugosoannulata, play an important role in the transformation of soil organic matter, including the degradation of pollutants. Information on the mechanisms of degradation of xenobiotics by these fungi and their ligninolytic enzymes is still limited. The degradative activity of the soil-inhabiting basidiomycete S. rugosoannulata towards four-rings PAHs was revealed. Fluorene and 2-carboxybenzaldehyde have been identified as products of degradation of fluoranthene and pyrene. The laccase of S.

Morphological Characteristics of the Main Bud of the Embrio the Grains of Winter Rye

Authors: Stepanov S. A., Kargatova A. M.

The paper presents the results of a study of the morphology of the cone of growth and primordium of leaves of the main bud of the embryo grain of winter rye of some varieties from different breeding centers in Russia. The length of the cone of growth reached in the investigated varieties of rye from 51 to 89 μm. Some varieties of rye in the cone of growth of the embryonic shoot of the embryo grains indicated the embedding of the 4th phytomere in the form of a leaf rudiment.

Biodegradation of Synthetic Dyes Bacteria of the Genus Azospirillum

Authors: Vorobyova S. A., Kupryashina M. A., Ponomarova E. G., Peterson A. М., Nikitina V. E.

Synthetic dyes are the largest class of chemicals that have found their commercial use in various industries. The most used are azo and anthraquinone dyes. However, most of them are not only toxic, carcinogenic, and mutagenic, but also resistant to biological destruction. The aim of the work was the screening of azospirillum strains according to their ability to biodegrade azo dyes and anthraquinone dyes. 

Analysis of Protease Effect on Biofilm Structure of Azospirillum Brasilense Strain Sp245 and Its Flagellation-Defective mmsB1 and fabG1 Mutants

Authors: Ponomarova E. G., Telesheva E. M., Sinyakin D. N., Shelud’ko A. V., Filip’echeva Y. A., Ponomareva E. G.

Azospirillum bacteria are engaged in associative interactions with a wide range of plants. In this type of interaction, there formed no specialized structures like nodules, which are characteristic of the legume-Rhizobium symbiosis. The formation of biofilms by Azospirillum on the plant root surface can be important for the successful functioning of plant-microbe associations. Scarce data exist on the role of cell surface protein structures in the formation and stabilization of Azospirillum biofilms. It is known that as compared to A.

Anatomical Structure of Seed which Belong to Iris L. Species of Saratov Flora

Authors: Minjal М. S., Kasatkin M. Y., Boldyrev V. A.

In the Saratov region all species of Iris are listed in the regional Red Book, with this connection, to preserve these plants, it is necessary to introduce them in an extensive researches, protection and establishing them in the literature. special interest is the study of seeds due to the direct relation with the potential possibility of generative propagation of Iris species, the article presents the results of an anatomical study of the seeds of seven species which belong to Iris L. in Saratov region flora.

Cytotoxicity Evaluation of Ionic and Colloidal Gold on the Microalga Dunaliella Salina in Microplate Test System

Authors: Chumakov D. S., Golubev А. А., Konnova S. A., Dykman L. А., Bogatyrev V. А.

Gold nanoparticles are intensively studied in biomedicine. Evaluation of their biocompatibility is highly important. In aquatic systems, microalgae, being the primary producers, are the base of the trophic webs. They are the first target for most of the pollutants present in the medium. To study the toxicity of ionic and colloidal gold on the saltwater microalga Dunaliella salina. To describe several physicochemical characteristics of nanomaterials that affect their toxicological efficiency.

Morphofunctional Changes in Microcirculatory Vessels of Hypertensive Rats’ Cardiac Muscle under Stress

Authors: Uvarova i. A., Romanova T. P.

Investigations of disorders of the heart’s structure and functions at the early stages of development, elaboration of prophylactic measures preventing or decreasing the risk of myocardium in- farction with the presence of the hypertension disease continue to remain unconditionally topical up to now. The aim of this ex- perimental work lies in finding out the morphofunctional signs of cardiovascular lesions produced by stress factors with the presence of arterial hypertension.

WBС Formula as an Indicator of the Adaptation Status of Rural and Urban Residents

Authors: Sysoyeva L. A., Ovsyannikova N. N., Lyakhova O. L.

The paper shows the opportunities of using WBC formula to evaluate the adaptation status of urban and rural residents to the complex of socio-economic and environmental conditions of the region. It was found that the age of the second physiological chiasm of Oryol region’s children accounts for a period of 5–6 years, which cor- responds to the generally accepted standards. Physiological highly adaptive responses were recorded among 47.4% of adolescents, 67% of rural and 36.7% of urban residents.

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