Biology

The biological properties of bacteria-assiociants Myzus cerasi F. in territory of the Saratov region weree studied. 39 strains of bacteria were revealed. 66.6% of the strains were able to use glucose, 87.0% – fixed molecular nitrogen, 56.3% – had the ability to grow at 10 °C, 92.1% of the isolated cultures were alkalotolerant and grown at pH10. 

The study of acoustic signals from various representatives of insects, and in particular coleopterans, has a long history. In the Russian-language literature, systematic, faunistic and ecological studies on aquatic beetles, including those on the family Hydrophilidae, are widely known; however, studies on the bioacoustics of the coleopteran group are quite rare. The aim of our study was to study stress signals of two species of Hydrophilidae – Berosus frontifoveatus Kuwert, 1888 and Berosus spinosus Steven, 1808.

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding.

The Herbaspirillum lusitanum P6-12 strain containing the vector plasmid pJN105TurboGFP, which encodes the synthesis of the green fluorescent protein GFP, and which has resistance to the antibiotic gentamicin, was obtained by electroporation. The constructed strain of H. lusitanum P6-12 in cultural, morphological and biochemical properties did not differ from the original typical natural strain of H. lusitanum P6-12. On solid growth media, the recombinant strain formed yellow-green colonies, fluorescent under UV irradiation.