Search articles

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).

Influence of the Cultivation Temperature on Morphometric and Functional Indices of Growth Triticum durum L.

Authors: Korobko V. V., Shevlyagina О. F.

The object of the study was sprouts of durum wheat Triticum durum L. Cultivation was carried out at a temperature of 15 ± 1°C and 20 ± 2°C. To study the influence of the temperature factor on the development of the photosynthetic apparatus, the quantitative content of chlorophyll a, chlorophyll b, and carotenoids was determined. Some varieties, when subjected to a change in the temperature of cultivation, respond with a change in the indices determining their stress tolerance, namely the ratio of chlorophyll a and chlorophyll b, carotenoids and chlorophylls.

Epiphytic Lichens of Natural Park “Kumysnaya Polyana”

Authors: Kozyreva E. A., Boldyrev V. A.

The article provides information about the epiphytic lichen flora of the Natural Park “Kumysnaya Polyana” (NP), which is located in Saratov. It is a regional SPNA (Special Protected Natural Area). The researches were conducted in forest communities of NP in 2011–2016. Standard techniques were used in the collection and identification of lichens. An index of species specificity of lichens for forophytes was calculated. An annotated list consists of 37 species and includes the information about their preferential forophytes.

Optimization of Photodynamic Effects on Permeability of Cerebral Vessels

Authors: Semyachkina-Glushkovskaya O. V., Shirokov A. A., Novolokin N. A., Terskov A. V., Bodrova A. A., Shushunova N. A., Khorovodov A. P., Ulanova M. V., Agranovich I. M., Iskra T. D., Mamedova A. T.

Photodynamic treatment (PDT) causes a significant increase in the permeability of the blood-brain barrier (BBB) in healthy mice. Using different doses of laser radiation (635 nm, 10–40 J/cm2 ) and photosensitizer (5-aminolevulinic acid – 5-ALA, i.v.), we found the optimal PDT for the reversible opening of the BBB, exhibiting brain tissue recovery 3 days after PDT. Further increases in the laser radiation or 5-ALA doses have no amplifying effect on the BBB permeability, but are associated with severe damage of brain tissues.

Phenological Characteristics of Orthopterous Insects (Orthoptera) in the Volga-Kama State Nature Biosphere Reserve

Authors: Karmzina I. O., Shulaev N. V.

In this brief communication, the results of the research on the seasonal dynamics of orthopterous insects (Orthoptera) are discussed. The research was performed in the Volga-Kama State Nature Biosphere Reserve. Field sampling and observations were carried out for different biotopes of the nature reserve. The phenological characteristics of 26 background species of the families Tettigonioidea, Tetrigidae, and Acridoidea were revealed. The species under study were divided into five phenological groups: spring-summer, early summer, summer summer-late summer, and late summer.

Lymphatic Meningeal Role in Processes of Brain Clearing: in vivo Visualization

Authors: Duarte Torres E. N., Abdurashitov A. S., Namykin A. A., Shirokov A. A., Shushunova N. A., Sarantseva E. I., Semyachkina-Glushkovskaya O. V.

In the experiments on rats, Evans Blue dye and gold nanorods were injected into the brain parenchyma, followed by visualization with optical coherent tomography and a fluorescent microscopy. The results showed that meningeal lymphatics play an important role in the brain clearing, using markers introduced into the tissues. It has been shown that the deep cervical lymph node is the first anatomical “station” for the outflow of fluid from the brain. The data obtained shed light on the lymphatic mechanisms underlying the drainage and clearing of brain functions.

The Effect of Pollutants on the Emergence of Rifampicin Resistance in Bacteria of the Pseudomonas Genus

Authors: Seliverstova E. Y., Vyrostkov V. A., Yarovaya E. V., Kulikov M. P., Drobot V. Y., Sazykin I. S., Sazykina M. A.

The anthropogenic pressure on ecosystems may contribute to the spread of antibiotic resistance among bacteria in natural conditions. Due to this, the aim of this work was to study the impact of specific biocides, antibiotics, pesticides and heavy metals on the emergence of rifampicin resistant mutants of Pseudomonas putida. To assess the impact of toxic substances on the emergence of rifampicin-resistant mutants the studied substances and rifampicin in the final concentration of 100 μg/ml were added into nutrient medium before plating.

Development of Fluorescent Glioma in Rats under Pharmacological Modulation of Beta2-Adrenergic Receptor

Authors: Khorovodov A. P., Shirokov A. A., Novolokin N. A., Terskov A. V., Lauberts E. A., Mamedova A. T., Shushunova N. A., Agranovich I. M., Ulanova M. V., Bodrova A. A., Semyachkina-Glushkovskaya O. V.

This study shows the role of adrenergic mechanisms in the development of fluorescent glioma in rats and the impairment of the blood-brain barrier (BBB) permeability. The results demonstrate that the progression of glioma was accompanied by a gradual increase in the BBB permeability and an increased expression of vascular beta2-adrenoreceptors (B2-AR). The pharmacological blockade of B2-AR reduced the degree of BBB disruption, the migration of cancer cells and increased the survival of animals.

Bacteria Fouling in the Systems of Technical and Circulating Water Supply of Balakovo Nuclear Power Station

Authors: Kirilova A. V., Glinskaya E. V., Voronin M. Y., Saveleva A. S.

The article is devoted to the study of bacteria in the system of technical water supply of Balakovo nuclear power plant (BNPP). The work was carried out in 2017. The samples were taken at the following sites: backup diesel generator of the 3rd unit (røde’s-3), pumping station of feeding pond-cooler (PSFPC), coastal pump station of unit 3 (CPS-U3), coastal pumping station of power unit № 4 (CPSP-U4), turbine hall of power unit № 3 (TH-PU3), turbine hall of power unit № 4 (TH-PU4).

Preparation and Identification of the Molecular Properties of Alginate Synthesized by the Cultivation of Azotobacter vinelandii D-05

Authors: Shutova V. V., Rusyaeva A. B.

It is known that bacterial alginates are widely used in biomedicine as carriers in the immobilization of cells, enzymes and drugs. Their functional properties depend on their monomeric composition and molecular weight and vary depending on the source and the cultivation conditions. It was established that molasses (waste of sugar beet production) can be used as an effective and cheap source of nutrition for the growth of the bacterium Azotobacter vinelandii strain D-05 and the production of alginate. Submerged periodic cultivation of the producer on media of different composition was used.

Biometric Characteristics and Aerodynamic Properties of Pollen Grains of North American Pines under Conditions of the Southern Coast of the Crimea

Authors: Plugatar Y. V., Sakhno T. M.

The results of the study of biometric characteristics and aerodynamic properties of pollen of North American species: Pinus radiata D. Don, Pinus sabiniana Douglas, Pinus coulteri D. Don and autochthonous Pinus nigra subsp. pallasiana (Lamb.) Holmboe, grown on the Southern Coast of the Crimea (SCC) are presented. The differences in the size of the pollen grains and their individual parameters are revealed. The pollen of introduced species is characterized by large sizes in comparison with native species.

Developing the Algorithm of Increasing the Destructive Potential of Pseudomonas putida SU12 Native Strain Extracted From Phenol-Contaminated Soils

Authors: Ilyina N. A., Ksenofontova O. Y., Tretyakova S. E., Tikhomirova E. I.

The article presents the development of methodological approaches to increasing destructive potential of Pseudomonas putida SU12 native strain extracted from phenol-contaminated soils. The cyclic selective method on gradual increase of phenol concentration in optoelectronics environment in combination with intermediate stage of accumulative culture is described. The authors proposed a discrete, stepwise increase in the concentration of phenol in elective media depending on degree of its destruction.

Investigation of Interaction Between Silicon-Based Nanoparticles and HeLa Cells

Authors: Terin D. V., Bratashov D. N., Revzina E. M., Rokakh G. E.

This work is about the investigation of the interaction of porous silicon submicron particles with the HeLa cervical cancer cell line. Substrates of the porous silicon were obtained with the two-staged chemical method based on synthesis of silver nanoparticles from silver nitrate AgNO3 with the subsequent etching with fluoric acid HF in the presence of hydrogen peroxide H2O2 and mechanical separation of microparticles by shattering. The thickness of the obtained porous silicon layer measured by scanning electrom microscopy (SEM), was about 2 mkm.

Photodynamic Diagnostics of Stomach Cancer

Authors: Agranovich I. M., Khorovodov A. P., Mamedova A. T., Shnitenkova A. A., Shimanova V. E., Novolokin N. A., Vashenko T. S., Kanevsky M. V., Galitskaya A. A., Konnova S. A., Borisova E. G., Semyachkina-Glushkovskaya O. V.

Cancer of the stomach is the main cause of death among cancer diseases of the gastrointestinal tract. This is due to the late diagnosis. This study was aimed at developing new methods for diagnosis of gastric cancer using a photosensitizer 5-ALK / PIXIX. The experiments were carried out on white non-native rats with an average mass of 250 grams. The animals were divided into 2 groups: control and experimental (stress + nitrosamine diet).

Low Temperature Preservation of an Economically Important Cyanophyta Species (Spirulina, Arthrospira)

Authors: Petrukhina D. I.

Spirulina and Arthrospira cyanoprokaryota species include a wide variety of laboratory microorganism culture collections and their traditional storage by means of isolate passages does not provide long-term preservation. The rationale for this study is to evaluate the possibility of long-term low temperature storage of Spirulina subsalsa and Arthrospira platensis cyanoprokaryota. Cyanoprokaryota biomass was stored for 12 months at minus 80 °С. The cooling rate was minus 1 °С.

Impact of the Predator on Quality Indicators Grain of Soft Wheat

Authors: Kulevatova T. B., Zlobina L. N., Beketova G. A., Starichkova N. I.

The aim of this study was to identify the effect of precursor (vapor, winter wheat) on the non-traditional selective grain quality parameter intensity of spring common wheat. The parameters include dough development time, dough stability, energy absorbed by dough while kneading and starch retrogradation coefficient. The data have been obtained using a Mixolab device.

Modern Cadastre of Species of Tularemia Microbe Carriers Habitant in Tularemia Foci of Different Types, Situated in the Territory of Russia

Authors: Tarasov M. A., Porshakov A. M., Kazakova L. V., Kresova U. А., Romanov R. A., Sludsky A. A.

The tularemia microbe can be found in all landscape zones of the Northern hemisphere, and in the Russian Federation this infection has been detected in virtually all regions. It spreads beyond the polar circle up to the 71° parallel. From 100 to 400 cases of tularemia infection are registered in the Russian Federation annually, 75% of which are accounted for the Northern, Central and Siberian territories of the country. From time to time epidemic morbidity rates are reported. Presently, six types of natural tularemia foci are established in Russia.

Current Status of the Collection Fund of the Laboratory of Ornamental Plants of the Russian Academy of Sciences

Authors: Bondorina I. A., Kabanov A. V., Mamaeva N. A., Khokhlacheva J. A., Bumbeeva L. I.

The Collection Fund of the Laboratory of Ornamental Plants of the Main Botanical Garden named after N. V. Tsitsin of the Russian Academy of Sciences has currently 1055 species and varieties, as well as 4 995 varieties and garden forms. It was created on the basis of classical methods of introduction, such as (ecology)-geographical, phytocoenotic and the method of generic complexes. At the same time, the modern policy of forming the collection fund is aimed at creating not only large but also unique collections.

Study of the Morphological Features of the Cells of the Bacteria Yersinia pestis, Grown at Different Temperatures by Atomic Force Microscopy

Authors: Utkin D. V., Bulgakova E. G., Erokhin P. S., Kuznetsov O. S., Kuklev V. E., Osina N. A.

Atomic force microscopy in the study of bacterial cells allows the determination of such morphological parameters as: length, width, cell thickness, its perimeter, cross-sectional area, volume and root mean square roughness of the cell surface, which depends on the degree of laying of peptidoglycan and density of lipopolysaccharide. The aim of this work was to study the changes in the morphological parameters of the cell and the cell surface of bacteria when the temperature conditions of the culture medium change on the Y. pestis model.

Influence of Probiotic “Bifidumbacterin” on the State of Large Intestine Microbiocenosis, Activity of the Antioxidant System and Processes of Lipid Peroxidation in the Conditions of Gentamycin-Associated Dysbiosis and Anomalous Characteristics of the Magn

Authors: Verevkina N. N., Medvedeva O. A., Korolev V. A., Shevchenko A. V., Kalutsky P. V.

Disturbances in the composition of microbiocenoses are warnings of changes in the physiological status of the organism, associated with the inhibition of immunobiological protection of the organism, its allergization, chronic intoxication, and increased susceptibility to infectious diseases. Some studies confirm the effect of magnetic radiation on abnormal characteristics both on the viability and growth rate of bacteria, and on the activity of various systems, in particular the antioxidant system and the lipid peroxidation system.

Some Features of Growth of Etiolated Plants in the Aspect of Implementation of Donor-Acceptor Relations

Authors: Shevlyagina О. F., Korobko V. V.

The object of the study was sprouts of spring soft wheat Triticum aestivum L. Cultivation was carried out in a climatic chamber at a temperature of 18 ± 1° C. As a control, plants grown during the photoperiod day / night 16/8 were used. Some features of the growth and development of Triticum aestivum L. plants were revealed under etiolation conditions. It was established that differences in the length of the root system of etiolated and control seedlings are determined by the length of the roots of the upper tier.

Pages