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Study of Correlation between Growth Rate of Children and Monophtalate Concentrations in Urine

Authors: Zaitseva N. V., Ulanova Т. S., Karnazhitskaya Т. D., Zavernenkova Е. О.

The study presents the results of determining of phthalate metabolites in urine of pre-school and primary school children by HPLC/MS. The correlation between growth rate of children and the concentration of phthalate metabolites in their urine was examined. The detected range of phthalate metabolite concentrations (mono-methyl phthalate, mono-butyl phthalate, mono-ethylhexyl phthalate) in urine of children was 0.0004–0.012 mg/dm3.

Type Specimens of Alchemilla L., Corispermum L., Koeleria Pers., Onosma L. Kept in the Sprygin Herbarium of Penza State University (PKM)

Authors: Vasjukov V. M., Novikova L. A.

Sprygin Herbarium of Penza State University – the largest sci- entific Botanical collection with the international acronym of the PKM, founded in 1894 on the initiative of the largest naturalist Ivan Sprygin. In the collections of the Penza Herbarium contains about 170 000 herbarium sheets of vascular plants, mossy plants, algae, lichens and fungi, of which over 30 authentic samples. Since 2009, the Herbarium is the electronic cataloguing of the collection. Results of revision on the type of genus Alchemilla L. (Rosaceae), Corispermum L. (Chenopodiaceae), Koeleria Pers.

Features of Growth and Development of Wheat Seedlings (TriticumaestivumL.) under the Action of 2,4,6-triphenyl-3,5-dichloropyridine and 2,6-diphenyl-3-chloropyridine

Authors: Korobko V. V., Pchelintseva N. V., Luneva M. А., Samsonova Е. А.

A biological testing of new synthetic heterocyclic compounds – 2,4,6-triphenyl-3,5-dichloropyridine and 2,6-diphenyl-3-chloropyri- dine differing more halogen atoms and phenyl fragments of the pyri- dine ring. The compounds were first obtained at the Department of Organic and Bioorganic Chemistry National Research Saratov State University. The objects of the study were the seedlings of spring wheat TriticumaestivumL. The test solutions had a positive effect on the rate root-maintenance seedlings in all variants of the experiment.

Community of the Aerobic Bacteria-destructors of DDT as a Result of Natural and Artificial Selection

Authors: Andreev D. N., Buzmakov S. A., Demakov V. A., Farofonova V. V., Egorova D. О.

For 40 years the soil microflora of the Landscape Reserve «Osinsk Forest Summer House» (Perm region, Russia) was exposed to high concentrations of DDT. Soil from the blocks 11 and 32 were selected and used in the process of artificial selection of aerobic bacterial communities capable of degrading DDT. Selection was carried out in the laboratory in four stages. As a result, successional changes were recorded, accompanied by reduction in the number and diversity of morphological types of bacterial strains in microbial communities.

On the Study of the Genus Alchemilla L. (Rosaceae) in the Penza Region

Authors: Vasjukov V. M., Chkalov A. V.

As a result of critical revision, 37 species of the genus Alchemilla were revealed in the Penza region (Russia): A. acutiloba Opiz, A. atrifolia Zamelis, A. baltica G. Sam. ex Juz., A. breviloba H. Lindb., A. cheirochlora Juz., А. conglobata H. Lindb., А. cymatophylla Juz., А. decalvans Juz., А. exilis Juz., А. glabricaulis H. Lindb., А. hebescens Juz., A. heptagona Juz., A. hians Juz., А. hirsuticaulis H. Lindb., A. homoeophylla Juz., А. leiophylla Juz., А. lessingiana Juz., А. lindbergiana Juz., A. litwinowii Juz., A. macrescens Juz., А. micans Buser, A. mininzonii Czkalov, А.

Screening of Genus Azospirillum for their Ability to Produce Extracellular Lignin-Peroxidase and the Degradation of Model Lignin Compounds and Azo Dyes

Authors: Petrov S. V., Kupryashina M. A., Glinskaya E. V., Nikitina V. E., Ponomarova E. G., Vorobyova S. A.

Lignin peroxidase is one of the main enzymes of fungi decomposers of wood, that is capable to many non-specific oxidation of aromatic and polycyclic compounds. By the beginning of our research there were virtually no data of the bacteria’s ability to produce the lignin peroxidase. Rather recently lignin peroxidase activity was detected by us in the washouts from the surface of the bacterial cells and in intracellular extracts of bacteria from genus Azospirillum.

The Influence of Growth Conditions of Bacteria Azospirillum lipoferum Sp59b on the Biological Activity of Their Glycopolymers

Authors: Surkina A. K., Konnova S. A., Fedonenko Y. P.

It is well known that lipopolysaccharides (LPSs) from outer membranes and capsular polysaccharides of Gram-negative bacteria activate in- nate immune system of humans and mammals and can produce septic shock clinical signs. A beneficial effect of LPSs consists in moderate stimulation of production of endogenous mediators by immune system cells, thus increasing the resistance of the organism to infections. In this respect the development of non-toxic derivatives of bacterial glycopolymers with improved immunostimulary properties becomes urgent.

The Influence of Aqueous Dispersions of Metal Nanoparticles on the Adhesive Properties of the Standard and Clinical Strains of Staphylococcus aureus

Authors: Nechayeva O. V., Bespalova N. V., Glinskaya E. V., Shurshalova N. F., Shulgina T. A., Nechayeva O. V., Torgashova A. S.

The initial stage of interaction of infectious agents with micro-organisms cells is the process of adhesion. So there is a need to develop methods that reduce the adhesive activity of microorganisms. Aim: to study the impact of water and water dialyzed dispersion of silver and copper nanoparticles obtained by biochemical synthesis, on the adhesive properties of the standard and clinical strains of S. aureus. Using standard techniques for cells of S.

About the Biology of the Invasive Species Hordeum jubatum L. in Bashkir Trans-Urals

Authors: Abramova L. M., Baymurzina Z. M., Yanturin S. I.

The results of researches of 7 coenopopulations of North American invasivespeciesHordeumjubatumL.(Poaceae)inthe Trans-Urals of the Republic of Bashkortostan. Hordeum jubatum spreads on the steppe zone of the region. It dominates over other plants and it is share of participation in the community is 45,5–60,5%. The density of grass invasive species – 9–18 plants per 1 m2.Each plant produces 35 to 68 shoots, on average, is more than 600 shoots per 1 m2. The biomass of the type is no more than 0.25 kg/m2. Seed production is 39–54 pcs.

Brunnera – Rare Decorative Culture for Shadow Garden

Authors: Zhigunov O. Y., Karimova O. A., Anishchenko I. Y.

The assortment of plants for a shadow garden in Bashkortostan, and also regions of South Urals is insignificant, it is not enough data on features of biology of shade and shade-enduring plants. Among them there are species and sorts of Brunnera Stev. genus. Brunnera are decorative and deciduous and beautiful-blossoming plants with the long period of blossoming. Studying of some biological features (seasonal rhythm of growth and development, morphometric param- eters, introduced stability) of five sorts of B.

Expiratory Lung Volumes of Young People at Rest and Recovery Period after a Dosed Physical Load

Authors: Sevryukova G. A., Isupov I. B., Shmarina Y. G., Ryaskova K. A., Belova B. A.

The present research is devoted to the problems of a complex estimation of functional reserves of the respiratory system of young untrained persons staying in the different conditions of life. The lung function of healthy young female persons at rest and after dosed physical activity, which used the sample of Martin, was investigated by standard and forced methods of spirometry.

WBС Formula as an Indicator of the Adaptation Status of Rural and Urban Residents

Authors: Sysoyeva L. A., Ovsyannikova N. N., Lyakhova O. L.

The paper shows the opportunities of using WBC formula to evaluate the adaptation status of urban and rural residents to the complex of socio-economic and environmental conditions of the region. It was found that the age of the second physiological chiasm of Oryol region’s children accounts for a period of 5–6 years, which cor- responds to the generally accepted standards. Physiological highly adaptive responses were recorded among 47.4% of adolescents, 67% of rural and 36.7% of urban residents.

Morphofunctional Changes in Microcirculatory Vessels of Hypertensive Rats’ Cardiac Muscle under Stress

Authors: Uvarova i. A., Romanova T. P.

Investigations of disorders of the heart’s structure and functions at the early stages of development, elaboration of prophylactic measures preventing or decreasing the risk of myocardium in- farction with the presence of the hypertension disease continue to remain unconditionally topical up to now. The aim of this ex- perimental work lies in finding out the morphofunctional signs of cardiovascular lesions produced by stress factors with the presence of arterial hypertension.

Cytotoxicity Evaluation of Ionic and Colloidal Gold on the Microalga Dunaliella Salina in Microplate Test System

Authors: Chumakov D. S., Golubev А. А., Konnova S. A., Dykman L. А., Bogatyrev V. А.

Gold nanoparticles are intensively studied in biomedicine. Evaluation of their biocompatibility is highly important. In aquatic systems, microalgae, being the primary producers, are the base of the trophic webs. They are the first target for most of the pollutants present in the medium. To study the toxicity of ionic and colloidal gold on the saltwater microalga Dunaliella salina. To describe several physicochemical characteristics of nanomaterials that affect their toxicological efficiency.

Anatomical Structure of Seed which Belong to Iris L. Species of Saratov Flora

Authors: Minjal М. S., Kasatkin M. Y., Boldyrev V. A.

In the Saratov region all species of Iris are listed in the regional Red Book, with this connection, to preserve these plants, it is necessary to introduce them in an extensive researches, protection and establishing them in the literature. special interest is the study of seeds due to the direct relation with the potential possibility of generative propagation of Iris species, the article presents the results of an anatomical study of the seeds of seven species which belong to Iris L. in Saratov region flora.

Analysis of Protease Effect on Biofilm Structure of Azospirillum Brasilense Strain Sp245 and Its Flagellation-Defective mmsB1 and fabG1 Mutants

Authors: Ponomarova E. G., Telesheva E. M., Sinyakin D. N., Shelud’ko A. V., Filip’echeva Y. A., Ponomareva E. G.

Azospirillum bacteria are engaged in associative interactions with a wide range of plants. In this type of interaction, there formed no specialized structures like nodules, which are characteristic of the legume-Rhizobium symbiosis. The formation of biofilms by Azospirillum on the plant root surface can be important for the successful functioning of plant-microbe associations. Scarce data exist on the role of cell surface protein structures in the formation and stabilization of Azospirillum biofilms. It is known that as compared to A.

Biodegradation of Synthetic Dyes Bacteria of the Genus Azospirillum

Authors: Vorobyova S. A., Kupryashina M. A., Ponomarova E. G., Peterson A. М., Nikitina V. E.

Synthetic dyes are the largest class of chemicals that have found their commercial use in various industries. The most used are azo and anthraquinone dyes. However, most of them are not only toxic, carcinogenic, and mutagenic, but also resistant to biological destruction. The aim of the work was the screening of azospirillum strains according to their ability to biodegrade azo dyes and anthraquinone dyes. 

Morphological Characteristics of the Main Bud of the Embrio the Grains of Winter Rye

Authors: Stepanov S. A., Kargatova A. M.

The paper presents the results of a study of the morphology of the cone of growth and primordium of leaves of the main bud of the embryo grain of winter rye of some varieties from different breeding centers in Russia. The length of the cone of growth reached in the investigated varieties of rye from 51 to 89 μm. Some varieties of rye in the cone of growth of the embryonic shoot of the embryo grains indicated the embedding of the 4th phytomere in the form of a leaf rudiment.

The Involvement of Laccase of the Fungus Stropharia Rugosoannulata DSM 11372 in Pah Degradation

Authors: Balandina S. А., Pozdnyakova N. N., Turkovskaya O. V.

Soil-inhabiting ligninolytic basidiomycetes, such as Stropharia rugosoannulata, play an important role in the transformation of soil organic matter, including the degradation of pollutants. Information on the mechanisms of degradation of xenobiotics by these fungi and their ligninolytic enzymes is still limited. The degradative activity of the soil-inhabiting basidiomycete S. rugosoannulata towards four-rings PAHs was revealed. Fluorene and 2-carboxybenzaldehyde have been identified as products of degradation of fluoranthene and pyrene. The laccase of S.

Study of Phylogenetic Connections of Representatives of the Genus Mesobuthus (Arachnida: Scorpiones) on the Basis of Molecular DNA Analysis

Authors: Poverenniy N. N., Anikin V. V.

The aim of the research is to determine the phylogenetic connections of scorpion representatives of the genus Mesobuthus living in the Lower Volga region and their comparison with other taxa (subspecies and species of this genus). For this, scorpions were collected on the territory of the Lower Volga region. The nucleotide sequences of the collected specimens were deciphered and compared with the sequences of species of the genus Mesobuthus from other regions of the Palearctic. The phylogenetic connections of the investigated objects are determined.

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