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Taxonomic Composition of Ground Invertebrates and Their Ratio in Different Types of Deciduous Forests within Tobolsk District of Tyumen Region

Authors: Vazhenina N. V.

The composition and spatial distribution of the surface & litterdwelling mesofauna in leaved forests the indigenous terrace of the Irtysh river are analyzed on the basis materials of accounting for invertebrates by soil traps. The surveys were carried out in 2005–2010 in three types of forests – aspen forest, lime forest and birch forest, located within Tobolsk district of the Tyumen region (southern taiga, Western Siberia). As a result, 281 species of invertebrates were identified in forests, about 50% of which were in the families Carabidae, Staphylinidae and Linyphiidae.

The Beetles (Insecta: Coleoptera), Collected by the Light Traps with Super Bright LEDs on the Territory of Krasnodar

Authors: Sazhnev A. S., Rodionova E. Y.

The collections were realized out in June – September 2018 using the original light trap within the city of Krasnodar. The article contains lists of 74 species (1252 specimens) of Coleopteran from 15 families: Carabidae – 13 species, Hydrophilidae – 12, Dytiscidae – 6, Elateridae – 6, Curculionidae – 4, Coccinellidae – 3, Heteroceridae – 2, Tenebrionidae – 2, Chrysomelidae – 2, Spercheidae, Scirtidae, Cantharidae, Bostrichidae, Anthicidae and Cerambycidae – in 1 species.

Generic Spectrum in the Analysis of Flora in Samara-Ulyanovsk Volga Region

Authors: Ivanova A. V., Kostina N. V., Aristova M. A.

Consideration of the generic spectrum of flora is one of the elements of a general analysis of the flora of the study area. It allows to identify individual traits to a greater extent than the analysis of the family spectrum. The study of phytodiversity at the level of floras will allow in the future to clarify the patterns of their territorial location. Samara-Ulyanovsk Volga region is the territory within two administrative regions (Samara and Ulyanovsk) belongs to the Middle Volga region. It is very diverse in natural conditions.

Effect of the O-Antigen Chemical Structure in Different Subspecies of Francisella Tularensis on Immunological Reactions

Authors: Gorbatov A. А., Titareva G. M., Kravchenko T. B., Shaykhutinova R. Z., Gerasimov V. N., Mokrievich A. N., Firstova V. V.

The greatest diagnostic significance for tularemia is played by antibodies against lipopolysaccharide (LPS). Previously, it has been shown that specific antibodies that appear in the blood serum of laboratory animals infected with virulent strains and people who have recovered from tularemia can bind both to LPS Francisella tularensis and to LPS Francisella novicida, while after the vaccination specific antibodies to LPS F. novicida are absent. The aim of this work was to investigate the effect of the structure of the O-antigen of lipopolysaccharide F.

Polysaccharidebased Biological Activity of Extracts Isolated from Fruits of Crataegus Monogyna of Varying Degrees of Maturity

Authors: Laksaeva E. A., Davydov V. V., Kyarimov I. A., Kukhtenkova E. A., Arushanova V. V., Zlobina E. A.

The fruits of midland hawthorn (Cratae gusoxyacantha L.), as well as leaves and flowers have a complex composition. A water-soluble polysaccharide complex, flavonoids and saponins, and other biologically active compounds were revealed in them. Consequently, preparations made from the vegetable material of hawthorn have a high biological activity. A liquid extract of fruits and a tincture of hawthorn flowers, are widely used in medicine. These drugs are used for cardiovascular diseases, have a sedative and hypotensive effect.

Induction of Direct Organogenesis in the Culture of Mature Embryo in Maize

Authors: Humood B. M., Yudakova O. I.

Regeneration of plants in vitro through direct organogenesis allows the avoidance of somaclonal variation. The aim of our study was the induction of direct organogenesis in the culture of mature embryo in maize lines created at Saratov State University: ATTM (bm, wx, y), ATTM (bm, y), ATTM (bm, g, V-type CMS), AT-3 (4n). These lines are characterized by genetic predisposition to parthenogenesis. The mature embryos isolated from sterile grains were used as a primary explant.

Vitality Structure and Ontogenetic Strategy of DeLPHINIUM litwinowii (Ranunculaceae)

Authors: Bogoslov A. V., Kashin A. S., Shilova I. V., Kritskaya T. A., Parhomenko A. S., Grebenyuk L. V.

The article presents the results of a study of the vitality structure of four Delphinium litwinowii Sambuk coenopopulations from the Penza, Tambov and Voronezh regions in the period from 2017 to 2018. The most northern coenopopulation from Penza region in 2017 showed the strongest vitality, while the weakest was the Voronezh’ coenopopula- tion, located to the south, near the village of Vladimirovka. Most of the coenopopulations over the observation period are rated as prosperous; coenopopulation from the vicinity of the village of Vladimirovka is de- fined as depressive.

The Phytotesting of (thio)semicarbazones 2,4-diarylbicyclo[3.3.1]non-2-en-9-ones

Authors: Mironova N. V., Korobko V. V., Pchelintseva N. V., Krylatova J. G., Zhestovskaya E. S.

Biological testing of (thio) semicarbazones 2,4-diarylbicyclo [3.3.1] non2-en-9-ones, differing in the nature of aryl substituents and nucleophile, was carried out.The object of the study was seedlings of spring soft wheat Triticum aestivum L. of the Saratovskaya 36 variety. To assess the physiological activity of the tested compounds, we used a valuable analysis of the morphometric parameters of growth of the root system and shoots of the test and control plants and the quantitative content of chlorophyll a, chlorophyll b, and carotenoids in the first leaf.

Changes in Blood-Brain Barrier Permeability under the Influence of Loud Sound

Authors: Esma S. A., Abdurashitov A. S., Namykin A. A., Shirokov A. A., Lykova E. Y., Sarantseva E. I., Iskra T. D., Vodovozova E. L., Khorovodov A. P., Terskov A. V., Mamedova A. T., Agranovich I. M., Klimova M. M., Semyachkina-Glushkovskaya O. V.

The development of methods of drug delivery to the brain is one of the priorities of the therapy of most cerebral diseases. There are more than 70 technologies to overcome the blood-brain barrier (BBB), but many of them are not widely used in daily clinical practice, as they lead to negative consequences. Here we propose a new method of overcoming the BBB using loud sound (110 dB, 370 Hz, duration – 2 hours).

Antimicrobial Photodynamic Effects Using Coatings Based on Metal Nanoparticles (Ag, Au)

Authors: Tuchina E. S., Gvozdev G. A., Kosobudskiy I. D., Shih W., Tuchin V. V.

The combined effect of coatings based on nanoparticles of metals Ag, Au, ultraviolet (365 nm, UVR) and infrared (808 nm, IRR) radiation on Staphylococcus aureus 209 P was studied. Decrease in numbers was shown by 2% in the case of silver coatings and by 8% in the case of gold coatings after 1 h incubation. However, exposure of the IRR to bacterial suspensions caused the death of 43% of the population after 5 min, 86% of the population after 30 min.

Application of New Instrumental Methods for Evaluation of the Functional State of Cells of Francisella Tularensis in a Stressful Environment

Authors: Borisova S. V., Kuznetsova E. M., Erokhin P. S., Volokh O. A.

The structure of the cell surface reflects the functional state of the bacterium as a whole. The presence of specific features of cells affects the biophysical characteristics of their surface: charge, free energy and hydrophobicity. The interaction of bacteria with biomolecules and particles is also related to these surface characteristics. The aim of our work was to study the function of F. tularensis 15 NIIEG cells under cultivation under stress using electro-optical (EO) monitoring.

Assessment of the Impact of the Nanoparticles of Silver Stabilized by Polymeric Compounds on Survival of Strains of Staphylococcus aureus

Authors: Shulgina T. A., Glinskaya E. V., Nechayeva O. V., Torgashova A. S., Zubova K. V.

The antimicrobial activity of the nanoparticles of silver stabilized by various natural and synthetic polymeric compounds concerning reference and clinical strains of Staphylococcus aureus is studied. It is established that the greatest effectiveness was displayed by the silver nanoparticles stabilized by polyvinyl alcohol, carboxymethylcellulose and poliazolidinammonium, the modified iodine hydrate ions. The low sensitivity of the studied strains to action of the nanoparticles stabilized by an oleate of S.

The Coefficient of Replenishment for the Commercial Stock of Catfish from the Release of Its Juvenile by Weighing Batch of 2 Grams in the Reservoirs of the Volga-Caspian Fishery Basin

Authors: Ermolin V. P.

The practical value of the coefficient of commercial replenishment of fish stocks, due to the variety of its use in different areas of fisheries research, design work, restoration activities, and management decisions was highlighted. For many species of fish there is no information on the indicator under consideration. Catfish, one of the valuable commercial species, is included in this category.

Modern Cadastre of Vectors and Other Reservoirs of Tularemia Microbe Found in Tularemia Foci of Different Types, Situated in the Territory of Russia

Authors: Tarasov M. A., Porshakov A. M., Kazakova L. V., Kresova U. А., Romanov R. A., Sludsky A. A.

At the present stage of the studies only Ixodidae ticks can be classed by the types of foci, being the main blood-sucking vectors of the tularemia agent. Other blood-sucking arthropods, considering the species abundance and wide-spread occurrence and certain habitat versatility, can be tularemia vectors in several or even in all types of foci. These are the following: primarily, gamaside and argasid ticks, as well as bloodsucking insects.

Ontogenetic Structure and Population Status of the Relict Species of Hedysarum gmelinii Ledeb. in the Cis-Urals Republic of Bashkortostan

Authors: Abramova L. M., Mustafina A. N., Karimova O. A.

The results of studying the ontogenetic structure of coenopopulations of the relict species Hedysarum gmelinii Ledeb. in the Bashkortostan of Republic are presented. The investigated cenopopulations growing on slopes of different exposure, with a slope of 35°, is the main type of vegetation – Helictotrichon desertorum–Stipa korshinskyi petrophyte steppe, anthropogenic load is low or medium. The overall density of the coenopopulations of H. gmelinii varies from 1,7 to 12,2 ind./m2, an effective density from 1,5 to 4,6 ind./m2.

Genetic Polymorphism in Populations of Hieracium × robustum (Asteraceae) as Result of the Hallabrottet Aulacidea hieracii (Hymenoptera: Cynipidae) Influence

Authors: Kritskaya T. A., Kashin A. S., Anikin V. V., Nikelshparg M. I.

Based on Inter Simple Sequence Repeats (ISSR), we examined polymorphism in populations of Hieracium × robustum (Asteraceae) – the host of gall-making Aulacidea hieracii (Hymenoptera, Cynipidae). In each population, we studied H. × robustum specimens with and without stem galls induced by gall wasps. It was revealed that individual gallfree plants have half as much ISSR fragments as plants contaminated by galls. In agarose gel, ISSR fragments of the former have clear-cut patterns while patterns of gall-contaminated plants are shapeless.

Morphogenesis and Reproductive Ability Monocarpic Escape Hosta sieboldiana (Hook.) Engl. (Hostaceae) in the Conditions of the Novosibirsk Region

Authors: Sedelnikova L. L.

The results of studying the morphogenesis of monocarpic escape during the seasonal development of Hosta sieboldiana are presented. It is shown that organ-forming processes of generative organs occur in the spring-summer vegetation of the shoot. Generative shoot is formed at 75–76 of days to flowering. Established, that the cone of increase of the escape differentiates intrarenal II to VIII stages of organogenesis. The flowers are laid in the acropolis direction. Duration of flowering was 25–38 days, fruiting 29–30 days. Real seed productivity is 3–4 times lower than potential.

Changes in the Permeability of the Blood-Brain Barrier in the Development of Alzheimer’s Disease in Mice

Authors: Zinchenko E. M., Klimova M. M., Shirokov A. A., Novolokin N. A., Martinov D. V., Antonova T. S., Blohina I. A., Agranovich I. M., Terskov A. V., Semyachkina-Glushkovskaya O. V.

In our study on mice with an injection model of Alzheimer’s disease (AD), beta-amyloid detection in the brain tissue was evaluated using immunohistochemistry and confocal analysis. An accumulation of toxic protein was shown in different areas of the brain, predominantly in the cortex and in the hippocamp (place of beta-amyloid injection). Neurofunctional tests were conducted to confirm the development of AD.

Peculiarities of Spatial Distribution of the Bottom Fauna in the River Kan (Tributary of the Middle Yenisei, Krasnoyarsk Region)

Authors: Andrianova A. V., Karepova E. D., Lepyavko M. P.

In the rivers of the Yenisei basin, benthic invertebrates play a major role in feeding most valuable Siberian fish species. This report presents advanced material on zoobenthos of the river Kan (one of the major tributaries of the Yenisei in its middle course). Litho-psammo-rheophilic biocenosis of benthic invertebrates was the most developed, 99 species and forms were recorded there. The total number of zoobenthos was formed from mayflies, caddisflies and chironomids; biomass – from caddisflies and mayflies.

The Additional Data about Spring Beetles Fauna (Insecta: Coleoptera) of the Natural Monument «Rasskazan’ Lake» (Saratov Province)

Authors: Sazhnev A. S., Volodchenko A. N., Lavrentiev M. V., Trushnov D. A.

The present work is a continuation of the inventory of the coleopterofauna of the water-terrestrial ecotones of the nature monument «Rasskazan’ Lake» Balashov district of Saratov province, which began in 2017. The study concerned the spring fauna of the period of occurrence of the Khoper river in low water after floods and the beginning of the stagnation of the lake water level. In total, 137 species from 18 families were noted for the studied biotopes, of which 41 species were new, with – 7 species appearing for the first time for the territory of the Saratov province.

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