Search articles

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).

Peculiarities of the Structure of Azospirillum zeae and Azospirillum formosense Lipopolysaccharides

Authors: Potapova О. А., Sigida E. N., Konnova S. A.

Lipopolysaccharides (LPS) of plant-growth-promoting bacteria of the genus Azospirillum are involved in the initial steps of formation of the association with host plant. This work aimed compositional analysis of the LPS from Azospirillum zeae and Azospirillum formosense. The LPS were extracted from the cells by hot phenolwater solution. Biopolymer composition of the LPS was studied by colorimetric methods. Determination of the fatty acid composition of the lipid A was performed by GLC. O-deacylation of the LPS A.

To the Question about the Reciprocal Conformity of the Transition Zone in the Chest Leads ECG, Heart Rate and Cerebral Hemodynamics

Authors: Sevryukova G. A., Isupov I. B., Tovmasian L. A.

This work is devoted to research of features of the vegetative support of activity of young persons, their cerebral hemodynamics depending on the localization of the transition zone in the chest leads ECG in the process of performing active orthostatic test. Registration of ECG, parameters of cardiorhythmography, reoentsefalografii was carried out using the diagnostic complex «Valenta».

Polyembryony Phenomenon in Apomictic Maize Line in vivo and in vitro

Authors: Alatortseva Т. А., Apanasova N. V., Lobanova L. P.

The article is focused on studying of cytoembryological preconditions of polyembryony in the not pollinated ovary of the apomictic maize line АТ-1 under in vitro and in vivo conditions. For an assessment of a nutrient medium role in polyembryony induction a relative analysis of the embryo sacs in cultivated in vitro and native plants ovaries has been lead. Dynamics of formation in them an apomictic twin proem- bryo is traced. In the study line the cause of polyembryony can be either an independent divisions in derivatives of egg cell, or division in additional egg cells.

Gamaside Mites of the Small Mammals Habitant in the Krasnoarmeisky Locality of the Saratov Region

Authors: Porshakov A. M., Chekashov V. N., Zakharov К. S.

Gamaside mites have ancient, strong links to a number of pathogens of natural-focal diseases. They constitute a considerable part of ectoparasites living on small mammals. Ac- cording to literature data and own research results, the fauna of Gamaside mites in the Saratov Region includes 44 species. Objec- tive of the study was to investigate the current state of Gamaside mite fauna ecologically associated with rodents in the territory of the Krasnoarmeisk District of the Saratov Region. The research was conducted in September-October, 2010 and in August, 2012.

Study of the Polymorphism DEFB4A Gene in Individuals Suffering from Periodontal Inflammatory Diseases

Authors: Verkhovsky R. A., Glinskaya E. V.

Inflammatory periodontal disease is considered a mass dental disease, the etiology of which is not yet fully understood.

The Specifics of Tobacco Gametophyte Mutations Display Depending on Temperature

Authors: Lobanova L. P., Kolesova А. Y., Alatortseva Т. А., Nurusheva I. A.

The research of extreme low (10°С) and high (37°С) temperatures influence under in vitro conditions on phenotypic display of three mutations in Nicotiana tabacum L. with of a certain morphological type domination was conducted. The BG-141.4 line is character- ized by low-cellular embryo sacs formation, in the SG-27/4 line the trait of the increased number of nucleus and cells in embryo sacs exhibits, the line М-3 differs by high frequency of cenocyte low- cellular embryo sacs.

Photodynamic Effect of Red Laser Radiation on the Growth of Staphylococcus aureus Strains with the Use of Photoditazine

Authors: Egorova А. V., Brill G. E., Bugaeva I. O., Tuchina E. S., Nechayeva O. V.

The influence of red laser irradiation on the growth of colonies of Staphylococcus aureus and photodynamic effect of the photosensi- tizer Photoditazin were performed. It is established that the radiation of a semiconductor red laser (λ = 660 nm, 100 mW/cm2.) has a direct bacteriostatic effect on the growth of S. aureus. This effect on the standard strain is manifested only when relatively high doses are used (180 J/cm2). Inhibition of bacterial growth reaches 36%.

Pollen Grain Size Polymorphism in Late Tulip (Tulipa gesneriana L., 1753) Cenopopulations within the Territory of “Ivanovo Field” Natural Boundary

Authors: Belyachenko A. A., Belyachenko Y. А.

One of the large populations of the late tulip (Tulipa gesneriana L., 1753) grows in Fedorovsky district of the Saratov region on the territory of the regional “Ivanovo field” natural boundary where it is presented by three cenopopulations. The work is aimed at studying of mature pollen morphology of the given cenopopulation plants characterised by various variants of flower parts coloration. We analysed the dimension and other morphological features of the pollen fixed in 2012 when rich diversity of floral envelope coloration was registrated in the research area.

The Influence of Roof Space Arrangement on Rock Pigeon` Penetration Level (Columba livia Gmelin, 1789)

Authors: Kukhta А. Е., Moskvitin S. S., Bolshakova N. P., Chapkina Т. V.

The statistics of correlation of rock pigeon occurrence depending on its construction in the roof rooms were presented (Columba Livia Gmelin, 1789). The relevance of the study conditioned by the need of some anthropogenic objects protection (monuments, granaries, airports) against birds particularly from rock pigeon which forms numerous flocks near the points of residence. The problem of bird hazard is particularly topical for airports where the pigeon is one of the most dangerous for the aircraft.

Flow Cytometry Investigations Indicators Light Scattering in the Heterogeneous Population Immune System Cells from Mice Immunized with F1 Capsular Antigen, Yersinia pestis

Authors: Kozhevnikov Н. V., Kurylina А. F., Kravtsov А. L., Shchukovskaya Т. N., Bugorkova T. V.

Aim – heterogeneous populations of blood leukocytes and abdomi- nal cavity of mice immunized with a capsular antigen of a plague microbe. Male mice of the BALB / c line were used in the work. The reaction of the cells of the immunophagocytic system was assessed by flow cytometry, in terms of the intensity of direct and lateral light scattering, correlating with changes in the cell volume and intracellular granularity.

Conserved and Variable Regions of the Methanol Dehydrogenase Gene Among Halotolerant Representatives of Methylophaga Genus

Authors: Sharavin D. Y., Solomennyi А. P.

We investigated conserved and variable regions of the mxaF gene and amino acid sequence in methylotrophic strain M1K isolated from saline soil rhizosphere and belonging to Methylophaga genus. The significant heterogeneity was revealed in methano- and methylotrophic bacteria by method of Minimum Evolution. The ability of strain to influence indole-3-acetic acid synthesis from L-tryptophan and halotolerance are the arguments in favor of application in new agrotechnology.

Mosquitos (Diptera; Culicidae) of the Saratov Region

Authors: Knyazeva Т. V., Porshakov A. M., Zakharov К. S., Matrosov А. N., Tolokonnikova S. I., Chekashov V. N., Shilov М. М., Yakovlev S. A.

Described is the fauna of bloodsucking mosquitoes found in the Saratov Region. The article is based on the data collected dur- ing the field surveys in the flooded biotopes along the Volga and Don river basins and Kamysh-Samara enclosed lake basin, as well as on the literature data available. By 1970s, 31 species of mosquitoes have been spotted in the Region. The paper presents qualitative and quantitative changes over the 45-year period in the populations of bloodsucking mosquito, habitant in the flooded areas of different rivers.

Antimycotic Activity of Silver Nanoparticles Depending on the Stabilizer Used

Authors: Shulgina T. A., Nechayeva O. V., Glinskaya E. V., Torgashova A. S., Teslyuk D. A., Babailova A. V., Panfilova E. A.

In the last decade, a large number of factual data about the steadily increasing complications caused by gram-positive and gram-negative microorganisms, as well as caused by fungi of the genus Candida, have been obtained. The developed situation was a powerful stimulus for the search and creation of new products containing substances with pronounced chemical-physical properties and high biological activity. The modern and promising direction was the using of nano- technology, in particular, metallic nanoparticles.

Effect of N,O,S-contaning Heterocyclic Compounds on Growth of Root System of Triticum aestivum L.

Authors: Korobko V. V., Samsonova Е. А., Batalin S. D., Pchelintseva N. V., Luneva M. А.

Conducted biological testing of synthetic heterocyclic compounds: 2-benzoylmethyl-3-benzoyl-2-phenylaziridine, 2-benzoyl-3, 5-diphenyl-4-chlorofuran and 2-amino-4(1,3-diphenyl-2-chloropropene-1-one-3-yl)-5-phenyl-1,3-thiazole. The investigated compounds are members of three series of heterocyclic compounds and obtained at the Department of Organic and Bioorganic Chemistry National Research Saratov State University. The concentration of substances established by the molecular weight, in the three specific to physiologically active substances acting doses: 10-6 М, 10-9 М, 10-12 М.

Rhizosphere Microorganisms’ Collection of IBPPM RAS: Revision of Azospirillum Strains Based on 16S rRNA Gene Sequence Analysis

Authors: Turkovskaya O. V., Golubev S. N., Dubrovskaya, E. V.

The IBPPM Collection maintains unique pool of bacterial strains related to Azospirillum genus, notable for its biotechnological potential. Progressively developing systematics of this genus requires checking and clarifying the taxonomic position for long-term stored isolates in the collection. Consequently, the species membership was confirmed or defined in the tested Azospirillum strains using a comparative analysis of their full-length 16S rRNA genes combined with morphological, cultural, physiological and biochemical properties, as well as DNA-DNA hybridization data.

Special Features of Flowering in Some Clematis L. Species and Forms from Nikita Botanical Gardens Collection

Authors: Zubkova N. V.

In the studies of ornamental plants seasonal development, flowering rhythms are exceptionally important, since the main criteria for the ornamental value of plants used for landscaping, are the time and duration of their flowering. The aim of the present work was to identify special features of flowering in some Clematis L. species and forms from Nikita Botanical Gardens collection. The objects of this study were 16 taxa. The methodology was based on the principles of introductory studies. Phenological and mathematical methods were applied.

Translocation of Copper, Zinc, Iron and Manganese in the Cultivated Oat (Avena sativa L., 1753)

Authors: Petukhov А. S., Khritokhin N. А., Petukhova G. А., Kudryavtsev А. А.

Due to rising anthropogenic pressure, heavy metal pollution of the environment becomes the significant danger. This study is devoted to the investigation of Cu, Zn, Fe and Mn concentration in the oat and two types of soil, model polluted by metal sulfates. The method of atomic absorption spectroscopy was used. Cu concentration in mineral soil was much higher than Zn. When Cu and Zn were combined, also Fe and Mn, pairwise antagonism in mineral soil and in the oat was discovered. The oat accumulates Cu and Mn more actively than Zn and Fe.

Laser-induced Vasomotor Responses on Chorioallantoic Membrane

Authors: Postnov D. E.

The strength and the specific featu res of the vasomotor responses provide important information about the healthy state of the blood vessel, since it s contraction or dilation is caused by the activation or relaxation of vascular smooth muscle cells of the vascular wall and appears to be a universal response to the a number of physiological regulatory pathways, including humoral and neurogenic ones.

The Use of Malaise Trap for the Study of the Beetles Fauna (Insecta: Coleoptera) in the Territory of National Park «Khvalynsky» Saratov Province

Authors: Sazhnev A. S., Anikin V. V.

In the article results of use of Malaise trap for the study of the beetles local fauna (Insecta: Coleoptera) in the territory of National Park «Khvalynsky» is given. Compiled a list of 95 species from 32 families Coleoptera. Antherophagus pallens (Linnaeus, 1758) and Saperda octopunctata (Scopoli, 1772) are recorded for Saratov region in first time.

Pages